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group II (LPS group), this group was only assigned to establish the acute orchitis model by injecting 0.5 mL saline containing LPS (Sigma-Aldrich, MO, United States) at a dosage of 5 mg/kg intraperitoneally ( After 24 h of the last treatment, the rats were weighed, and blood samples were collected from the retroorbital venous plexus under anesthesia (ketamine, 50 mg/kg, and xylazine, 2.20 mg/kg) ( 2.4 Semen examination Semen collection was performed by longitudinally opening the cauda epididymis, and sperm motility was examined microscopically