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Preparing PT-141 nasal spray Why nasal spray: Avoids injections (needle-phobic) More convenient Slightly faster onset (30-60 min quicker) Lower bioavailability (~30% vs ~100% injection) Need higher dose to compensate Nasal spray supplies needed: Empty nasal spray bottle (10-15ml) Reconstituted PT-141 (higher concentration recommended) Syringe for transferring Small funnel (optional) Concentration for nasal spray: Higher concentration better (less volume per dose) Reconstitute 10mg PT-141 in 1ml water = 10mg/ml Or even 10mg in 0.5ml = 20mg/ml (very concentrated) Allows smaller spray volume Fewer sprays needed per dose Nasal spray dosing: Inject: 1.5mg dose Nasal equivalent: 3-5mg (lower absorption) 0.3ml of 10mg/ml concentration = 3mg nasal 0.5ml of 10mg/ml = 5mg nasal Typical: 2-3 sprays per nostril (0.1ml per spray) See complete PT-141 nasal spray guide for men for detailed protocols

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12), suggesting relatively high biosafety of BaO 2