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Use a “hard and fast” approach when pushing the needle (bevel and shaft) into the skin, going in at a 90 degree angle (see video below)

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Conclusion Proper storage of lyophilised IGF-1 LR3 at 2-8°C (or -20°C for extended storage), careful reconstitution using bacteriostatic water with proper aseptic technique, and responsible handling of reconstituted solutions are critical for maintaining research integrity