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ADA activity was assayed under saturated substrate conditions at 37 °C in a reaction mixture containing 2 mM adenosine, 10 mU xanthine oxidases, 10 mU purine nucleoside phosphorylase, and 50 mM sodium phosphate (pH 7.4), in a total volume of 200 μL, of which 1 μL was the cell lysate
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