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The Arrhenius equation governing reaction rates shows that every 10°C increase in storage temperature roughly doubles the degradation rate—meaning a serum left on a bathroom counter at 30°C degrades four times faster than one refrigerated at 4°C
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Milligram:5mg
for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein
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