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Lentivirus production and infection First, 293T cells were cotransfected with lentiviral plasmids along with packaging plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) using polyethylenimine transfection reagent (Polysciences, 9002-98-6)

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Science 249 (4975), 1438–1441

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