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Cells were harvested, resuspended in either Buffered Methanol-complex Medium (BMMY: 1% yeast extract, (Difco) 2% peptone, 100 mM potassium phosphate pH 6.0, 1.34% YNB, 0.4 mg/L biotin, 0.5% methanol, Sintorgan) or buffered minimal methanol medium containing histidine (Sigma) (BMMH: 100 mM potassium phosphate pH 6.0, 1.34% YNB, 0.4 mg/L biotin, 4 mg/L histidine, 0.5% methanol) to an initial DO 600nm = 1.0, and incubated at 28 °C with shaking at 250 rpm in flasks covered with microporous tape sheets for better oxygenation

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This compound has not been evaluated by the FDA and is not intended to diagnose, treat, cure, or prevent any disease