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The nutrient deficiency can also activate the NOD-like receptor (NLR) family and pyrin domain-containing 3 (NLRP3) inflammasome and induce IL-1β secretion in high fat diet-induced atherosclerosis animal model 48 , whereas zinc supplementation can inhibit NLRP3 inflammasome activation through its antioxidant effects in an in vitro study 49
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The primary outcomes assessed included pain intensity measured via the Visual Analog Scale (VAS), serum levels of vitamin D, functional capacity evaluated through the Cooper 12-Minute Walk Test, and overall quality of life as determined by the SF-36 instrument
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