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In brief, dissected cerebral cortices were dissociated and grown in DMEM (Fisher 11960-044) with 1 mM sodium pyruvate (Corning 11360070), 20 mM HEPES (Gibco 15630-080), 100 U/ml PenStrep (Life Technologies 15140122), 10% fetal bovine serum (FBS, Atlanta 337 Biologicals S11150), and 5 µg/ml insulin
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A Cochrane systematic review of NAC for COPD exacerbations (39 RCTs, n=7,436) found that NAC and other mucolytics significantly reduced the frequency of acute exacerbations (OR 0.75, 95% CI 0.62-0.92) (Poole et al., Cochrane Database Syst Rev, 2019) [43]
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