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The main causes of fatigue are: (i) accumulation of protons in the muscle cell, reducing the pH and affecting the activity of enzymes, such as phosphofructokinase, (ii) depletion of energy sources (e.g., phosphocreatine and glycogen) for the continuity of the exercise, (iii) accumulation of ammonia (toxic metabolite) in the blood and tissues [2,3,4], (iv) oxidative stress, (v) muscle damage [1] and (vi) changes in neurotransmitter synthesis, such as the increase in serotonin and the decrease in dopamine [5], which may cause a state of tiredness, sleep, and lethargy during prolonged exercises [33]
