ghk cu peptide interactions GHK-CU Does Not Contain_Peanuts Size:4 oz bpc 157 umbrella labs
bpc 157 umbrella labs péptidos bpc 157 y tb 500 BPC-157 (Body Protection Compound-157)
(15)制备 (15) Preparation Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin 在步骤(14)的 In step (14) of Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin中,加入脱帽试剂(20%哌啶的DMF溶液),常温反应5分钟,抽干,重新加入脱帽剂常温反应20分钟,用异丙醇洗涤3次,DMF洗涤3次,重蒸过的DMF洗涤2次,抽干,加入Fmoc-Gly-OH0.8g,DIEA0.4ml,TBTU0.56g,HOBT0.2g,DMF10ml常温反应1.5小时,用真空泵抽干,用异丙醇洗涤3次,DMF洗涤3次,重蒸过的DMF洗涤2次,抽干,获得Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin; In Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin, add decapping reagent (20 %piperidine in DMF solution), reacted at normal temperature for 5 minutes, drained, added capping agent and reacted at normal temperature for 20 minutes, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with DMF evaporated, drained, Add 0.8g of Fmoc-Gly-OH, 0.4ml of DIEA, 0.56g of TBTU, 0.2g of HOBT, and 10ml of DMF to react at room temperature for 1.5 hours, dry it with a vacuum pump, wash 3 times with isopropanol, and wash 3 times with DMF
Does Not Contain_Peanuts
Size:4 oz
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