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Immunohistochemical labeling was visualized with Alexa Fluor™ 488- (RRID: AB_143165, Thermo Fisher Scientific Inc., 1:200) or Cy3- (RRID: AB_2307351, Jackson ImmunoResearch Laboratories, Inc., 1:200) conjugated secondary antibodies, and images were obtained using a laser scanning confocal microscope EZ-C1