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In addition, very high levels of antibodies are introduced to the skin in clinical settings when therapeutic antibodies such as trastuzumab (anti-HER2), adalimumab (anti-TNF), denosumab (anti-RANKL), and ustekinumab (anti–IL-12 and anti–IL-23) are administered s.c

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Complex-driven H 2 O 2 production was measured using Amplex Red (Thermo Fisher), according to the manufacturer’s instructions but with the following modifications: after 24 h of BPM31510 treatment, the media were aspirated and the cells were washed with 1× MAS buffer